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UB Flow Booster Kit - hover-over.png
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UB Flow Booster Kit - hover-over.png

UB Flow Booster Kit

from €225.00

Add further signal amplification to your flow cytometry assay with the UB Flow Booster Kit. Designed for use with the UB Flow Core Kit, it increases staining index a further 5-fold with one additional staining step. Together, Core and Booster provide a 50–150-fold higher staining index than conventional fluorophore-conjugated streptavidin or secondary antibodies.*

The Booster Pack extends the Core workflow for assays requiring improved detection of low-abundance antigens. Available in AF488, AF647, and Cy3B (PE alternative).

Requires a compatible UB Flow Core Kit. Not for standalone use. Also available as UB Flow Core + Booster Bundle.

Download the UB Flow Booster Kit staining protocol.


*Performance varies by assay and product configuration. Comparisons refer to the corresponding conventional fluorescent detection reagent.

For Research Use Only. Not for use in diagnostic procedures.

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Add further signal amplification to your flow cytometry assay with the UB Flow Booster Kit. Designed for use with the UB Flow Core Kit, it increases staining index a further 5-fold with one additional staining step. Together, Core and Booster provide a 50–150-fold higher staining index than conventional fluorophore-conjugated streptavidin or secondary antibodies.*

The Booster Pack extends the Core workflow for assays requiring improved detection of low-abundance antigens. Available in AF488, AF647, and Cy3B (PE alternative).

Requires a compatible UB Flow Core Kit. Not for standalone use. Also available as UB Flow Core + Booster Bundle.

Download the UB Flow Booster Kit staining protocol.


*Performance varies by assay and product configuration. Comparisons refer to the corresponding conventional fluorescent detection reagent.

For Research Use Only. Not for use in diagnostic procedures.

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Enhanced CD123 detection on OCI-AML5 cells by flow cytometry

The UB Flow Core Kit is designed to deliver strong fluorescence signals with minimal background for detecting low-abundance antigens by flow cytometry. We tested it on OCI-AML5 cells with low CD123 surface expression - approximately 5,000 molecules per cell, measured using an antigen quantification kit. Cells were stained with a biotinylated anti-CD123 antibody and analyzed using AF488 fluorescence detection.

Compared with conventional fluorescent streptavidin (SA), the Core Kit increased background-subtracted fluorescence (ΔMFI) approximately 15-fold. Adding the UB Flow Booster Kit increased this to approximately 100-fold, while kit background remained close to unstained cells. This combination of strong signal and low background yielded staining indices of 78 with Core and 440 with Core + Booster, substantially exceeding the staining index of 5 achieved with conventional fluorescent streptavidin.