UB Flow Core + Booster Bundle

from €540.00

Maximum signal amplification in one package.

The Core + Booster Bundle combines both signal amplification steps for demanding flow cytometry applications - ideal for low-abundance targets, challenging antibodies, and experiments where maximum population separation is the goal.

Combine antigen detection and additional signal amplification with the UB Flow Core + Booster Bundle. This flow cytometry bundle pairs the Core Kit with a compatible Booster Kit to deliver a 50–150-fold higher staining index than conventional fluorophore-conjugated streptavidin or secondary antibodies.*

The bundle includes Core reagents, Booster reagents, and a dedicated blocking solution. Follow the simple Core staining protocol, then add one Booster step to further improve detection of low-abundance antigens.

Choose AF488, AF647, or Cy3B (PE alternative), with streptavidin or polyclonal anti-mouse, anti-human, or anti-rabbit IgG (H+L) binders.

Download the UB Flow Booster Kit staining protocol.

*Performance varies by assay and product configuration. Comparisons refer to the corresponding conventional fluorescent detection reagent.

For Research Use Only. Not for use in diagnostic procedures.

Fluorophore:

Binder:

Size:

Maximum signal amplification in one package.

The Core + Booster Bundle combines both signal amplification steps for demanding flow cytometry applications - ideal for low-abundance targets, challenging antibodies, and experiments where maximum population separation is the goal.

Combine antigen detection and additional signal amplification with the UB Flow Core + Booster Bundle. This flow cytometry bundle pairs the Core Kit with a compatible Booster Kit to deliver a 50–150-fold higher staining index than conventional fluorophore-conjugated streptavidin or secondary antibodies.*

The bundle includes Core reagents, Booster reagents, and a dedicated blocking solution. Follow the simple Core staining protocol, then add one Booster step to further improve detection of low-abundance antigens.

Choose AF488, AF647, or Cy3B (PE alternative), with streptavidin or polyclonal anti-mouse, anti-human, or anti-rabbit IgG (H+L) binders.

Download the UB Flow Booster Kit staining protocol.

*Performance varies by assay and product configuration. Comparisons refer to the corresponding conventional fluorescent detection reagent.

For Research Use Only. Not for use in diagnostic procedures.

You can also pay by invoice. If you prefer invoice payment to credit card payment, request a quote below. We also accept purchase orders.

Request Quote

Enhanced CD123 detection on OCI-AML5 cells by flow cytometry

The UB Flow Core Kit is designed to deliver strong fluorescence signals with minimal background for detecting low-abundance antigens by flow cytometry. We tested it on OCI-AML5 cells with low CD123 surface expression - approximately 5,000 molecules per cell, measured using an antigen quantification kit. Cells were stained with a biotinylated anti-CD123 antibody and analyzed using AF488 fluorescence detection.

Compared with conventional fluorescent streptavidin (SA), the Core Kit increased background-subtracted fluorescence (ΔMFI) approximately 15-fold. Adding the UB Flow Booster Kit increased this to approximately 100-fold, while kit background remained close to unstained cells. This combination of strong signal and low background yielded staining indices of 78 with Core and 440 with Core + Booster, substantially exceeding the staining index of 5 achieved with conventional fluorescent streptavidin.

UB Flow Core + Booster Bundle

You Might Also Like